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Part:BBa_J72004

Designed by: John Anderson   Group: Anderson Lab   (2008-07-16)

{phi80-att} phage integration site

Plasmids containing the phi80 att site can be recombined into the E. coli genome with the aid of the phi80 integrase protein. Usually, this is expressed from a helper plasmid. See PMID: 11591683 for details. In house, this part is referred to as pBca9145-Bca1183 {Phi80-att}.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 164
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 411
    Illegal SpeI site found at 164
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 164
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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